Interplay between ABA and phospholipases A2 and D in the response of citrus fruit to postharvest dehydration
Identifieur interne : 001118 ( Main/Exploration ); précédent : 001117; suivant : 001119Interplay between ABA and phospholipases A2 and D in the response of citrus fruit to postharvest dehydration
Auteurs : Paco Romero [Espagne] ; M Nica Gandia [Espagne] ; Fernando Alferez [Espagne]Source :
- Plant physiology and biochemistry : (Paris) [ 0981-9428 ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Déshydratation, Agrume.
English descriptors
- KwdEn :
Abstract
The interplay between abscisic acid (ABA) and phospholipases A2 and D (PLA2 and PLD) in the response of citrus fruit to water stress was investigated during postharvest by using an ABA-deficient mutant from 'Navelate' orange named 'Pinalate'. Fruit from both varieties harvested at two different maturation stages (mature-green and full-mature) were subjected to prolonged water loss inducing stem-end rind breakdown (SERB) in full-mature fruit. Treatment with PLA2 inhibitor aristolochic acid (AT) and PLD inhibitor lysophosphatidylethanolamine (LPE) reduced the disorder in both varieties, suggesting that phospholipid metabolism is involved in citrus peel quality. Expression of CsPLDa and CsPLDβ, and CssPLA2α and CssPLA2β was studied by real-time RT-PCR during water stress and in response to ABA. CsPLDa expression increased in mature-green fruit from 'Navelate' but not in 'Pinalate' and ABA did not counteract this effect. ABA enhanced repression of CsPLDa in full-mature fruit. CsPLDβ gene expression decreased in mature-green 'Pinalate', remained unchanged in 'Navelate' and was induced in full-mature fruit from both varieties. CssPLA2α expression increased in mature-green fruit from both varieties whereas in full-mature fruit only increased in 'Navelate'. CssPLA2β expression increased in mature-green flavedo from both varieties, but in full-mature fruit remained steady in 'Navelate' and barely increased in 'Pinalate' fruit. ABA reduced expression in both after prolonged storage. Responsiveness to ABA increased with maturation. Our results show interplay between PLA2 and PLD and suggest that ABA action is upstream phospholipase activation. Response to ABA during water stress in citrus is regulated during fruit maturation and involves membrane phospholipid degradation.
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Agustín Escardino, 7</s1><s2>Paterna, Valencia 46980</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Gandia, M Nica" sort="Gandia, M Nica" uniqKey="Gandia M" first="M Nica" last="Gandia">M Nica Gandia</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Av. 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Agustín Escardino, 7</s1><s2>Paterna, Valencia 46980</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Plant physiology and biochemistry : (Paris)</title><title level="j" type="abbreviated">Plant physiol. biochem. : (Paris)</title><idno type="ISSN">0981-9428</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant physiology and biochemistry : (Paris)</title><title level="j" type="abbreviated">Plant physiol. biochem. : (Paris)</title><idno type="ISSN">0981-9428</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic acid</term><term>Citrus fruit</term><term>Citrus sinensis</term><term>Dehydration</term><term>Plant physiology</term><term>Postharvest</term><term>Production quality</term><term>Response</term><term>Water stress</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Réponse</term><term>Après récolte</term><term>Déshydratation</term><term>Qualité production</term><term>Stress hydrique</term><term>Citrus sinensis</term><term>Acide abscissique</term><term>Agrume</term><term>Physiologie végétale</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Déshydratation</term><term>Agrume</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The interplay between abscisic acid (ABA) and phospholipases A<sub>2</sub> and D (PLA<sub>2</sub> and PLD) in the response of citrus fruit to water stress was investigated during postharvest by using an ABA-deficient mutant from 'Navelate' orange named 'Pinalate'. Fruit from both varieties harvested at two different maturation stages (mature-green and full-mature) were subjected to prolonged water loss inducing stem-end rind breakdown (SERB) in full-mature fruit. Treatment with PLA<sub>2</sub> inhibitor aristolochic acid (AT) and PLD inhibitor lysophosphatidylethanolamine (LPE) reduced the disorder in both varieties, suggesting that phospholipid metabolism is involved in citrus peel quality. Expression of CsPLDa and CsPLDβ, and CssPLA<sub>2</sub>α and CssPLA<sub>2</sub>β was studied by real-time RT-PCR during water stress and in response to ABA. CsPLDa expression increased in mature-green fruit from 'Navelate' but not in 'Pinalate' and ABA did not counteract this effect. ABA enhanced repression of CsPLDa in full-mature fruit. CsPLDβ gene expression decreased in mature-green 'Pinalate', remained unchanged in 'Navelate' and was induced in full-mature fruit from both varieties. CssPLA<sub>2</sub>α expression increased in mature-green fruit from both varieties whereas in full-mature fruit only increased in 'Navelate'. CssPLA<sub>2</sub>β expression increased in mature-green flavedo from both varieties, but in full-mature fruit remained steady in 'Navelate' and barely increased in 'Pinalate' fruit. ABA reduced expression in both after prolonged storage. Responsiveness to ABA increased with maturation. Our results show interplay between PLA<sub>2</sub> and PLD and suggest that ABA action is upstream phospholipase activation. Response to ABA during water stress in citrus is regulated during fruit maturation and involves membrane phospholipid degradation.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté valencienne</li></region></list><tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Romero, Paco" sort="Romero, Paco" uniqKey="Romero P" first="Paco" last="Romero">Paco Romero</name></region><name sortKey="Alferez, Fernando" sort="Alferez, Fernando" uniqKey="Alferez F" first="Fernando" last="Alferez">Fernando Alferez</name><name sortKey="Gandia, M Nica" sort="Gandia, M Nica" uniqKey="Gandia M" first="M Nica" last="Gandia">M Nica Gandia</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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